1-naphthaleneacetic acid promoted in vitro adventitious rooting frequency up to 73.3%, whereas indole-3-butyric acid was not effective. The use of 6-benzylaminopurine in combination with 1-naphthaleneacetic acid proved superior to the application of thidiazuron which induced a frequent formation of short and fasciated shoots. Transfer of living shoots onto modified woody plant medium with the pH adjusted to 6.8–7.0 led to vigorous growth of multiple shoot cultures without any loss of multiplication rates or decreased vitality for several years. Stored seed should be cold stratified for 3 - 4 months and sown as early as possible in the year 164. The seed must be separated from the fruit flesh since this contains germination inhibitors 80, 164. Grown for small yellow flowers in late winter and red berries in autumn. Propagation Seed - best sown as soon as it is ripe in a cold frame or in an outdoors seedbed if there is sufficient seed 80, 113. Deciduous, open, spreading shrub or small tree. Cornus mas (also called Cornelian cherry) Genus Cornus. Later on, growth ceased, shoot tip necrosis appeared and shoot cultures died. Cornus mas Garden Plant Information Name. Multiple shoot cultures, grown on basal woody plant medium with the pH adjusted to 5.6–5.7 and supplemented with 6-benzylaminopurine in combination with 1-naphthaleneacetic acid, were capable of continuous axillary and adventitious shoot proliferation up to 1 year. That’s tough to accomplish, especially with fruit that ripens in early October. After that, the seeds need an additional 90 to 120 days of cold stratification (below 39 degrees). Sprouting axillary buds sampled from a mature 27-year-old shrub of Cornus mas ‘Macrocarpa’ were used as starting material for in vitro culture establishment. Sources vary a bit, but most say that cornelian cherry seeds require somewhere between 90 and 120 days of warm moist stratification (above 68 degrees f). Micropropagation of mature Cornus mas ‘Macrocarpa’ Micropropagation of mature Cornus mas ‘Macrocarpa’